Food has become an important part of the tourist experience and, accordingly, a special food tourism, known as gastro tourism, has developed. Through food, tourists can learn about the cultural values of the local community. On the other hand, the local community can use gastronomic tourism to promote cultural heritage and development. The most developed gastronomic region in Croatia is Istria, which offers a high-quality range of local products. In this thesis, the potential of truffle as a basis for the further development of gastronomic tourism in Istria was analyzed. For research purposes two methodologies were used: a survey and an interview. In addition, the interest of respondents in gastronomic tourism of Istria and truffles in the gastronomic offer of Istria was studied. The results show a high opinion about the offer of Istria and Istria as a gastronomic and tourist destination. The research confirmed the hypothesis. Furthermore, the obtained results confirm that Istria is a gastronomic region with a developed and quality offer and shows that there is an interest of both foreign and domestic tourists for truffles. To achieve the wanted results, it is recommended to educate the local population and tourists and to invest in the promotion of local products. Also, Istria should continue with its variety of tourist attractions, high-quality products, and top service because these are the factors that motivate tourists to visit.