The theme of this Master thesis is the analysis of the influence that non-verbal communication has on the process of business negotiation. The aim of this paper is to explore business negotiation as one of the key business processes, and (non-verbal) communication as an integral part of this process. Business in the 21st century is marked by constant and rapid changes in a highly competitive enviroment. Therefore, special attention is provided to skills and know-how that contribute to higher profits. One of these skills is non-verbal communication, and applying it as a part of the business negotiation process. This thesis presents basic types, strategies and characteristics of business negotiations. Moreover, it presents a historical overview of communication types and analysis of non-verbal communication. Thereafter, it examines its classification and impact in the overall communication process, followed by a more detailed analysis of its impact in business negotiation. A survey was conducted on a sample of 85 respondents. The instrument employed was a questionnaire consisting of 18 questions examining recognition of non-verbal signs in everyday life and business negotiations. The survey resulted to useful information that confirmed the initial assumption stating the importance of non-verbal communication in the overall communication process. However, respondents did not recognize non-verbal communication signs as a part of the business negotiation when prompted. Mastering non-verbal communication is directly related to the positive outcome of negotiations. This thesis argues that the skillful negotiator with sufficient knowledge of non-verbal communication will be more successful in business negotiations. As such, multiple benefits are gained both for the individual and for the company.