LGBT tourism is a form of niche tourism that gains its significance with time. While many European and world destinations have designated their businesses according to that segment, Croatia still hesitates to develop this form of tourism. The purpose of this paper was to examine attitudes about the development of LGBT tourism in Zagreb as a potential destination for its possible development. For the research purposes, two focus groups were conducted, one of which was composed of people who did not have significant contact with the subject of work, and the second of only LGBT people, after which a comparison of the results was made. By analyzing the results, it was concluded that respondents believe society is still not ready enough for the significant development of LGBT tourism in Zagreb. Given the nature of the research method, the conclusions of the respondents can not be generalized and further research on a larger sample of subjects should be made. The author thinks this work has given the foundation and contribution to future research in this area.