Entrepreneurship in the Republic of Croatia is made up of the largest number of small and medium-sized enterprises, that is, 98% of them are included in these categories. With Croatia's entry into the European Union, entrepreneurship in the Republic of Croatia gains special importance, because Croatia is guaranteed access to EU funds and grants intended for the improvement of the entrepreneurship system in the Republic of Croatia. Given that before joining the EU, Croatia had to harmonize its national legislation with the acquis of the EU, it is important to mention that this also applied to business regulations. In this final paper, the emphasis is on the analysis of the use of EU funds for the purpose of encouraging and developing entrepreneurship in the Republic of Croatia, and for the purposes of the research, two phases of research were conducted based on convenient sample of respondents: telephone interviewing and survey research via Google Forms questionnaires with randomly selected companies that are available on the Internet portal of the Zagreb Stock Exchange, all with the aim of drawing a conclusion on the use of the EU funds. The research was conducted on a sample of 46 companies, and based on the results of the research, it is concluded that respondents’ companies that were founded before Croatia's entry into the EU were financed from their own funds, while respondents’ companies that were founded after 2013 used various incentives and funds from EU funds. Furthermore, the results of the research indicate that the largest number of respondents’ companies have successfully used funds from EU funds in their operations so far, which ultimately had a positive effect on the company's operations and the improvement of primary activities.