The paper analyses the influences of organizational support factors on employee motivation in the implementation of new business activities for the parent company. Entrepreneurship within the existing organizational structures is defined as the process of redistribution and new combinations of existing resources and the creation of new products / services, technologies, and organizational units. In the theory of entrepreneurship within the organization, the entrepreneurial employee stands out as the most important resource of the organization that turns its idea into added value and thus provides the necessary profit and competitive advantage for their company. The paper highlights and analyses the importance of motivating employees to implement new business activities. Employee satisfaction as a driver of entrepreneurial activities is especially treated. Employee satisfaction is seen as a prerequisite for motivation and the result of motivation, in a cause-and-effect relationship. The basic motivating factors are the need to achieve, accepting risks and setting one's own goals. Finally, through the elements of management support, freedom in business, emphasizing entrepreneurial activities, availability of time and control of organizational boundaries, the analysed influence of organizational support factors on employee motivation and consequently undertaking new business activities is defined.