Nautical tourism in the Republic of Croatia is one of the key segments of the tourism industry, significantly contributing to economic development, particularly in coastal areas. This thesis explores how human resource management within companies engaged in nautical tourism impacts the sector’s competitiveness, adaptation to seasonal fluctuations, changes in tourist preferences, and global challenges. The focus is on analyzing human resource management practices using the example of Navigare Yachting d.o.o., one of Croatia’s leading charter companies, with the aim of understanding how human resources can be strategically utilized to enhance service quality and customer satisfaction. The thesis consists of five key chapters that address various aspects of nautical tourism and human resource management. The introductory part outlines the fundamental research guidelines, followed by an analysis of the role of human resources in Croatian tourism, with a special emphasis on their importance for industry competitiveness and sustainable development. A particular focus is placed on nautical tourism, its specificities, and challenges, including sustainability aspects and adaptation to market changes. The practical part of the thesis involves an analysis of real-life examples from the operations of Navigare Yachting d.o.o., with a focus on human resource management within this context. Through research, this thesis offers a comprehensive insight into current practices and challenges in managing human resources in nautical tourism in the Republic of Croatia, highlighting the importance of human resources in developing competitive advantages, providing high-quality services, and maintaining ecological standards within this dynamic and growing industry.