The aim of theses paper is based on infrastructural support for women entrepreneurs, primarily on the example of the Virtual Women's Entrepreneurial Center. This work describes basic features of female entrepreneurship, infrastructural support in the Republic of Croatia, on the one side a theoretical framework for entrepreneurial infrastructural support, and on the other side women's entrepreneurship, and unavoidable examples of good practice from all around the world. Republic of Croatia is one of a few countries which has developed Strategy for Development of Women Entrepreneurship in the Republic of Croatia 2014-2020. with an aim to increase competitiveness of the small business and to effectively accelerate the economic potential of women. Virtual Women's Entrepreneurial Center is the first center of that kind, not only in Croatia, but also throughout The Southeastern Europe. The Center is opened to promote entrepreneurship, but primarily it tries to help women in the current women entrepreneurs, or those who might become entrepreneurs in future. Since the opening from January 1st, 2016, there are currently 2300 female members and 84 male members. Furthermore, theses paper contains a survey which represents user's overviews and efficacy of the Virtual Women's Entrepreneurial Center, which was conducted by a questionnaire composed of 17 questions on a sample of 33 respondents and also, interview with founder of the Center. The results showed that members which have used services provided by the Virtual Women's Business Center were extremely satisfied with level of service and that all needed services and information were provided in a timely manner and in accordance with their needs.