Gender discrimination is a major problem in the world of employment, with serious consequences for women in the labor market. Statistical data clearly indicate a significant gender gap, whereby women make up a larger share of the unemployed than men and often face numerous challenges in professional development. Women in the workplace often suffer various forms of discrimination due to their gender, marital status and motherhood, and traditional stereotypes further worsen their position and opportunities for advancement. Although there are laws that protect pregnant women, women still face difficulties when returning from maternity leave. Employers' prejudices can contribute to the perception of women as less valuable workers or as sexual objects, which further worsens their status in the workplace. Also, harmonizing business and family life is a significant challenge for many women. The paper deals with the topic of discrimination against women in the workplace in the Republic of Croatia, investigating legal aspects, including laws on gender equality and measures to combat discrimination. The aim of the research was to examine in detail the perception of working women about discrimination in the Croatian labor market. Using the quantitative method we surveyed 147 women from different sectors and positions. The results of the research showed that discrimination against women in employment and work still exists. Men are often privileged in employment and promotion, while women face additional barriers, including questions about maternity leave plans, which points to gender stereotypes and inequalities.