This paper investigates and analyzes the current biggest problems and obstacles of start-up entrepreneurs in business incubators in Virovitica-Podravina County. It has been determined how to help both start-up entrepreneurs and business incubators. Secondary and primary sources were used in the preparation of the diploma thesis, and examples of good practice of business incubators were studied. The empirical part of the thesis is based on information collected through two surveys on a sample of 42 respondents and a semi-structured in-depth interview with the Deputy Director of the Incubator Network of Virovitica-Podravina County. The conditions in business incubators in Virovitica-Podravina County are excellent, given that measures of free rent were recently introduced and both office spaces and production facilities are newly equipped. The County is trying to make doing business easier for all start-up entrepreneurs by providing financial support, and in addition to the County’s, financial support is also given by cities where business incubators are located. The biggest obstacles and problems are the lack of labor in Virovitica-Podravina County, a myriad of administrative barriers regarding entrepreneurial activities, and low economic activity of the private and agricultural sectors in terms of using modern technologies offered on the market. The conditions for the development of entrepreneurial ventures are excellent but some improvements need to be made, such as additional networking of institutions at the state level, improved mentoring services, conferences, seminars, roundtables, and similar activities to attract younger people to business incubators. The conclusion drawn is that the conditions for start-up entrepreneurs in business incubators in Virovitica-Podravina County are excellent, but there is always room for improvement and business incubators in Virovitica-Podravina County are ready to use these opportunities for progress.